Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish

Huifang Xu; Xuxia Tang; Jingjing Chen; Ya Shi; Jun Liu; Shengya Guo; Jiali Zhou; Chunqi Li; Jing He Zhou

doi:doi:10.11648/j.ijast.20210503.15

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Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish

Huifang Xu, Xuxia Tang, Jingjing Chen, Ya Shi, Jun Liu, Shengya Guo, Jiali Zhou, Chunqi Li, Jing He Zhou

Published in International Journal of Animal Science and Technology (Volume 5, Issue 3)

Received: 2 September 2021 Accepted: 22 September 2021 Published: 30 September 2021

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Abstract

Tripterygium glycosides (TG) is isolated from an extensively used traditional Chinese medicine herb tripterygium roots and has been extensively used in the treatment of rheumatoid arthritis, nephrotic syndrome, hyperthyroidism and other diseases due to its anti-inflammatory and immunosuppressive effects. Hearing toxicity has been recently associated with TG use in human patients. In this study, authors assessed hearing toxicity and possible molecular toxic mechanisms of TG in a whole animal model. The maximum non-lethal concentration (MNLC) of TG on the zebrafish was 21 mg/L. TG induced zebrafish hair cell loss in a dose-dependent manner (p<0.001), and the saccular otolith size reduction when treated at MNLC (p<0.01). TG treatment resulted in sound-stimulated zebrafish movement reduction (p<0.001); and the rollover zebrafish percentages were elevated as TG treatment concentrations moved up. Following TG treatment, mRNA levels of the zebrafish hearing organ development genes eya1 and val were remarkably downregulated, and the expression of apoptosis-associated genes bax and caspase3 was significantly enhanced (p<0.05). These findings confirm the hearing toxicity of TG and suggest its toxic mechanisms probably are through suppressing hearing cell development and promoting hearing cell apoptosis. Authors recommend zebrafish assay as a quick and reliable screening test of hearing toxicity for drugs and health products.

Published in	International Journal of Animal Science and Technology (Volume 5, Issue 3)
DOI	10.11648/j.ijast.20210503.15
Page(s)	79-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Tripterygium glycosides, Hearing Toxicity, Hair Cell, Zebrafish

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APA Style

Huifang Xu, Xuxia Tang, Jingjing Chen, Ya Shi, Jun Liu, et al. (2021). Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. International Journal of Animal Science and Technology, 5(3), 79-86. https://doi.org/10.11648/j.ijast.20210503.15

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ACS Style

Huifang Xu; Xuxia Tang; Jingjing Chen; Ya Shi; Jun Liu, et al. Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. Int. J. Anim. Sci. Technol. 2021, 5(3), 79-86. doi: 10.11648/j.ijast.20210503.15

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AMA Style

Huifang Xu, Xuxia Tang, Jingjing Chen, Ya Shi, Jun Liu, et al. Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. Int J Anim Sci Technol. 2021;5(3):79-86. doi: 10.11648/j.ijast.20210503.15

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@article{10.11648/j.ijast.20210503.15,
  author = {Huifang Xu and Xuxia Tang and Jingjing Chen and Ya Shi and Jun Liu and Shengya Guo and Jiali Zhou and Chunqi Li and Jing He Zhou},
  title = {Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish},
  journal = {International Journal of Animal Science and Technology},
  volume = {5},
  number = {3},
  pages = {79-86},
  doi = {10.11648/j.ijast.20210503.15},
  url = {https://doi.org/10.11648/j.ijast.20210503.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20210503.15},
  abstract = {Tripterygium glycosides (TG) is isolated from an extensively used traditional Chinese medicine herb tripterygium roots and has been extensively used in the treatment of rheumatoid arthritis, nephrotic syndrome, hyperthyroidism and other diseases due to its anti-inflammatory and immunosuppressive effects. Hearing toxicity has been recently associated with TG use in human patients. In this study, authors assessed hearing toxicity and possible molecular toxic mechanisms of TG in a whole animal model. The maximum non-lethal concentration (MNLC) of TG on the zebrafish was 21 mg/L. TG induced zebrafish hair cell loss in a dose-dependent manner (pTG treatment resulted in sound-stimulated zebrafish movement reduction (pTG treatment concentrations moved up. Following TG treatment, mRNA levels of the zebrafish hearing organ development genes eya1 and val were remarkably downregulated, and the expression of apoptosis-associated genes bax and caspase3 was significantly enhanced (pTG and suggest its toxic mechanisms probably are through suppressing hearing cell development and promoting hearing cell apoptosis. Authors recommend zebrafish assay as a quick and reliable screening test of hearing toxicity for drugs and health products.},
 year = {2021}
}

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TY  - JOUR
T1  - Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish
AU  - Huifang Xu
AU  - Xuxia Tang
AU  - Jingjing Chen
AU  - Ya Shi
AU  - Jun Liu
AU  - Shengya Guo
AU  - Jiali Zhou
AU  - Chunqi Li
AU  - Jing He Zhou
Y1  - 2021/09/30
PY  - 2021
N1  - https://doi.org/10.11648/j.ijast.20210503.15
DO  - 10.11648/j.ijast.20210503.15
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20210503.15
AB  - Tripterygium glycosides (TG) is isolated from an extensively used traditional Chinese medicine herb tripterygium roots and has been extensively used in the treatment of rheumatoid arthritis, nephrotic syndrome, hyperthyroidism and other diseases due to its anti-inflammatory and immunosuppressive effects. Hearing toxicity has been recently associated with TG use in human patients. In this study, authors assessed hearing toxicity and possible molecular toxic mechanisms of TG in a whole animal model. The maximum non-lethal concentration (MNLC) of TG on the zebrafish was 21 mg/L. TG induced zebrafish hair cell loss in a dose-dependent manner (pTG treatment resulted in sound-stimulated zebrafish movement reduction (pTG treatment concentrations moved up. Following TG treatment, mRNA levels of the zebrafish hearing organ development genes eya1 and val were remarkably downregulated, and the expression of apoptosis-associated genes bax and caspase3 was significantly enhanced (pTG and suggest its toxic mechanisms probably are through suppressing hearing cell development and promoting hearing cell apoptosis. Authors recommend zebrafish assay as a quick and reliable screening test of hearing toxicity for drugs and health products.
VL  - 5
IS  - 3
ER  -

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Author Information

Huifang Xu

Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China
Xuxia Tang

Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China
Jingjing Chen

Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China
Ya Shi

Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China
Jun Liu

Otolaryngology Department, Zhejiang Provincial Hospital of Traditional Chinese Medical, Hangzhou, China
Shengya Guo

Hunter Biotechnology, Hangzhou, China
Jiali Zhou

Hunter Biotechnology, Hangzhou, China
Chunqi Li

Hunter Biotechnology, Hangzhou, China
Jing He Zhou

Department of Plastic Surgery, Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine, Hangzhou, China

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APA Style

Huifang Xu, Xuxia Tang, Jingjing Chen, Ya Shi, Jun Liu, et al. (2021). Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. International Journal of Animal Science and Technology, 5(3), 79-86. https://doi.org/10.11648/j.ijast.20210503.15

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ACS Style

Huifang Xu; Xuxia Tang; Jingjing Chen; Ya Shi; Jun Liu, et al. Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. Int. J. Anim. Sci. Technol. 2021, 5(3), 79-86. doi: 10.11648/j.ijast.20210503.15

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AMA Style

Huifang Xu, Xuxia Tang, Jingjing Chen, Ya Shi, Jun Liu, et al. Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish. Int J Anim Sci Technol. 2021;5(3):79-86. doi: 10.11648/j.ijast.20210503.15

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@article{10.11648/j.ijast.20210503.15,
  author = {Huifang Xu and Xuxia Tang and Jingjing Chen and Ya Shi and Jun Liu and Shengya Guo and Jiali Zhou and Chunqi Li and Jing He Zhou},
  title = {Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish},
  journal = {International Journal of Animal Science and Technology},
  volume = {5},
  number = {3},
  pages = {79-86},
  doi = {10.11648/j.ijast.20210503.15},
  url = {https://doi.org/10.11648/j.ijast.20210503.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20210503.15},
  abstract = {Tripterygium glycosides (TG) is isolated from an extensively used traditional Chinese medicine herb tripterygium roots and has been extensively used in the treatment of rheumatoid arthritis, nephrotic syndrome, hyperthyroidism and other diseases due to its anti-inflammatory and immunosuppressive effects. Hearing toxicity has been recently associated with TG use in human patients. In this study, authors assessed hearing toxicity and possible molecular toxic mechanisms of TG in a whole animal model. The maximum non-lethal concentration (MNLC) of TG on the zebrafish was 21 mg/L. TG induced zebrafish hair cell loss in a dose-dependent manner (pTG treatment resulted in sound-stimulated zebrafish movement reduction (pTG treatment concentrations moved up. Following TG treatment, mRNA levels of the zebrafish hearing organ development genes eya1 and val were remarkably downregulated, and the expression of apoptosis-associated genes bax and caspase3 was significantly enhanced (pTG and suggest its toxic mechanisms probably are through suppressing hearing cell development and promoting hearing cell apoptosis. Authors recommend zebrafish assay as a quick and reliable screening test of hearing toxicity for drugs and health products.},
 year = {2021}
}

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TY  - JOUR
T1  - Hearing Toxicity Induced by Tripterygium Glycosides in Zebrafish
AU  - Huifang Xu
AU  - Xuxia Tang
AU  - Jingjing Chen
AU  - Ya Shi
AU  - Jun Liu
AU  - Shengya Guo
AU  - Jiali Zhou
AU  - Chunqi Li
AU  - Jing He Zhou
Y1  - 2021/09/30
PY  - 2021
N1  - https://doi.org/10.11648/j.ijast.20210503.15
DO  - 10.11648/j.ijast.20210503.15
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20210503.15
AB  - Tripterygium glycosides (TG) is isolated from an extensively used traditional Chinese medicine herb tripterygium roots and has been extensively used in the treatment of rheumatoid arthritis, nephrotic syndrome, hyperthyroidism and other diseases due to its anti-inflammatory and immunosuppressive effects. Hearing toxicity has been recently associated with TG use in human patients. In this study, authors assessed hearing toxicity and possible molecular toxic mechanisms of TG in a whole animal model. The maximum non-lethal concentration (MNLC) of TG on the zebrafish was 21 mg/L. TG induced zebrafish hair cell loss in a dose-dependent manner (pTG treatment resulted in sound-stimulated zebrafish movement reduction (pTG treatment concentrations moved up. Following TG treatment, mRNA levels of the zebrafish hearing organ development genes eya1 and val were remarkably downregulated, and the expression of apoptosis-associated genes bax and caspase3 was significantly enhanced (pTG and suggest its toxic mechanisms probably are through suppressing hearing cell development and promoting hearing cell apoptosis. Authors recommend zebrafish assay as a quick and reliable screening test of hearing toxicity for drugs and health products.
VL  - 5
IS  - 3
ER  -

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