Volume 2, Issue 1, March 2018, Page: 6-13
Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms
Kanenus Dereje Dereje, Wama Hagalo Districts of East Wollega Zone, Oromia Region, Nekemte, Ethiopia
Abriham Kebede, School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia
Nigus Abebe, College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Yobsan Tamiru, School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia
Received: Nov. 17, 2017;       Accepted: Nov. 29, 2017;       Published: Jan. 17, 2018
DOI: 10.11648/j.ijast.20180201.12      View  1355      Downloads  130
Abstract
A cross-sectional study was conducted from November 2014 to April 2015 to determine the prevalence of bovine mastitis, isolation of mastitis causing bacteria’s and characterize antimicrobial susceptibility pattern at Holeta Agricultural Research Centre (HARC) Dairy Farms. Purposive sampling technique was employed and all lactating cows were involved in the study. Among 186 lactating cows consists of 92 Jersey as well as 94 cross breed (Boran X Holistein Fresian) were included in the study. Among selected cows, 131 (70.43%) cows were identified as bovine mastitis positive and out of which 10 (5.37%) had clinical mastitis and 121 (65.05%) had subclinical mastitis. The different types of bacterial species isolated in present study includes S. aureus (30.93%), staphylococcus other than S. aureus (14.43%), Str. agalactiae (5.15%), Str. Dysgalactiae (5.15%), Str. Uberis (12.37%), C. bovis (13.40%), E. coli (6.18%), P. aerogenosa (10.3%) and K. pneumonie (2.05%). Antimicrobial susceptibility testing revealed that 94% and 78.6% of total isolates were susceptible to gentamycin and trimethoprim-sulfamethoxazole, respectively which was followed by erythromycin and tetracycline with susceptibility rate of 73.8% and 69%, respectively. However, penicillin had shown susceptibility of 55.9% which was the least effective among the drugs used. In conclusion, different types of mastitis causing pathogens with variable rate of susceptibility to antimicrobials were able to identify. Therefore, antimicrobial therapy should be used after isolation of the suspected pathogen to avoid emergence of drug resistance.
Keywords
Antimicrobial, Dairy, Isolation, Mastitis, Susceptibility, HARC
To cite this article
Kanenus Dereje Dereje, Abriham Kebede, Nigus Abebe, Yobsan Tamiru, Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms, International Journal of Animal Science and Technology. Vol. 2, No. 1, 2018, pp. 6-13. doi: 10.11648/j.ijast.20180201.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Akers, R. M. (2002): Lactation and the Mammary Gland. Iowa State Press, Ames, Iowa, USA, 2002. Pp: 278.
[2]
Almaw, G., Molla, W. and. Melaku A (2009): Prevalence of bovine subclinical mastitis in Gondar town and surrounding areas, Ethiopia. Livestock Ressearch Rural Development, 21 (7).
[3]
Al-Majali, A. M., K. M. Al-Qudah, Y. H. Al-Tarazi and O. F. Al-Rawashdeh (2008): Risk factors associated with camel brucellosis in Jordan. Tropical Animal Health Production 40: 193-200.
[4]
Ararsa, D., Tadele, T., and Aster, Y. (2014): Prevalence of clinical and sub-clinical mastitis on cross bred dairy cows at Holleta Agricultural Research Center, Central Ethiopia. Journal of Veterinary Medicine and Animal Health 6 (1): 13-17.
[5]
Atyaib, N., Vodjgani M, Gharagozloo F, Bahonar A (2006): Prevalence of bacterial mastitis in cattle from the farms around Tehran. Iranian Journal of Veterinary Research 3 (16): 76-79.
[6]
Bedada, B., and A. Hiko, (2011): Mastitis and antimicrobial susceptibility test at Asella, Dairy. Oromia Regional state, Ethiopia. Journal of Microbiology and Antimicrobial 3: 228-23.
[7]
Birhanu, A., Diriba, L., and Iyob, I. (2013): Study of bovine mastitis in asella government dairy farm of Oromia regional state, South Eastern Ethiopia. International Journal of Current Research and Academic Review 1 (2): 134-145.
[8]
Biruk, D., and Shemeles, A. (2015): Isolation and identification of major bacterial pathogen from clinical mastitis cow raw milk in Addis Ababa, Ethiopia. Academic Journal of Animal Disease 4 (1): 44-51.
[9]
Bishi, A. B. (1998): Cross sectional and longitudinal prospective study of bovine clinical subclinical mastitis in per-urban and urban dairy production in Addis Ababa. MSc thesis, Addis Ababa university, Faculty of veterinary medicine, Debra Zeit, Ethiopia.
[10]
Bitew M, Tafere A, Tolosa T (2010). Study on Bovine Mastitis in Dairy Farms of Bahir Dar and its Environs. Journal of Animal and Veterinary Advances, 9 (23): 2912-2917.
[11]
Bradley, A. J. (2002). Bovine Mastitis: an Evolving disease. Veterinary Journal 163: 1-13.
[12]
Capuco, A. V. et al. (1992): Increased susceptibility to intra mammary infection following removal of teat canal keratin. Journal of Dairy Science 75: 2126–2130.
[13]
Central Statistical Agency (CSA) (2014): Livestock and Livestock Characteristics, Agricultural Sample Survey. Statistician Bulletin, 2 (537): 107.
[14]
Dabash, H., Petros, A., and Fekadu, A. (2014): Prevalence and Identification of Bacterial Pathogens Causing Bovine Mastitis from Crossbred of Dairy Cows in North Showa Zone of Ethiopia. Global Veterinaria 13 (2): 189-195.
[15]
Dego, O. K., and G. F. Tarek (2003): Bovine mastitis in selected areas of southern Ethiopia. Tropical Animal Health and Production 35 (3): 197-205.
[16]
Demelash, B., Etana, D. and B. Fekadu, (2005): Prevalence and Risk Factors of Mastitis in Lactating. International Journal of Applied Research in Veterinary Medicine 21: 3.
[17]
Enyew, G. A. (2004). A cross-sectional study of bovine mastitis in and around bahirdar and antibiotic resistance patterns of major pathogens. MSc thesis Addis Ababa University Debre Zeit, Ethiopia.
[18]
Eriskine, R. J. (2001): Intramuscular administration of ceftiofursodiu versus intra mmamary infusion of penicillin/novobiocin for treatment of Streptococcus agalactiae mastitis in dairy cows. Journal of American Veterinary Medical Association 208: 258-260.
[19]
FAO, (2009): Crop Diversification and Marketing Development Project, Interim Report. Addis Ababa, Ethiopia.
[20]
Fadlelmoula, A., Fahr, R., Anacker, G, Swalve, H (2007). The Management Practices Associated with Prevalence and Risk Factors of Mastitis in Large Scale Dairy Farms in Thuringia-Germany 1: Environmental Factors Associated with Prevalence of mastitis. Australian Journal of Basic and Applied science 1 (4): 619-624.
[21]
Fekadu, K. (1995): Survey on Prevalence of Bovine Mastitis and the Predominant Causative Agent. In Proceeding of 9 Conference of Ethiopia Veterinary Association, Addis Ababa Ethiopia, Pp: 101-111.
[22]
Girma, D. (2010): Study on prevalence of mastitis on cross Breed Dairy cows Around Holeta areas, West Shewa Zone of Ethiopia. Global Veterinaria 5 (69): 31 8-323.
[23]
International Dairy Federation (1987): Bovine Mastitis: Definition and Guidelines for Diagnosis. Bulletin of the International Dairy Federation Pp. 211, 24.
[24]
Hamann, J. (2005): Diagnosis of Mastitis and Indicators of Milk Quality. IN: Hogeveen, H. (Ed.) Proceedings of 4th IDF International Dairy Conference: Mastitis in Dairy Production - Current Knowledge and Future Solutions. Pp. 82-90.
[25]
Hogeveen, H, Huijps, K., and Lam, T. (2011): Economic aspects of mastitis: new developments. New Zealand Veterinary Journal, 59 (1): 16-23.
[26]
Husien, N., Yehualashet, T., and Tilahun, G. (1999): Prevalence of Mastitis in Different Local and Exotic and Breed of Milking Cows. Ethiopian Journal of Agricultural Science, 16: 53-60.
[27]
Junaidu, A. U., Salilu, M. D., Jambuwal, F. M., Magoji, A. A., and Jaafaru, S. (2011). Prevalence of mastitis in lactating cows in some selected commercial dairy farms in Sokoto metropolis. Advances in Applied Research, 2 (2): 290-294.
[28]
Korhonen, H., and L. Kaartinen (1995): Changes in the composition of milk induced by mastits. In: The bovine udder and mastitis. Sandholm, M., T. Honkanen-Buzalski, L. Kaartinen and S. Pyörälä (eds.), University of Helsinki, Finland Pp. 76-82.
[29]
Kerro, O., Tareke, F., (2003): Bovine mastitis in selected areas of Southern Ethiopia. Tropical Animal Health Production 35: 197-205.
[30]
Lemma, M., Kassa, T., and Tegegne, A. (2001): Clinically manifested major problems cross breed dairy in urban and pre-urban production system in central highland of Ethiopia. Tropical Animal Health and Production 33: 85-89.
[31]
Mekibib, B., Furgasa, M., Abunna, F., Megersa, B., and Regassa, A. (2010): Bovine Mastitis: Prevalence, Risk Factors and Major Pathogens in Dairy Farms of Holeta Town, Central Ethiopia. Veterinary world, 3 (9): 397-403.
[32]
Moges, N., Asfaw, Y., and Belihu, K. (2011): A Cross Sectional Study on the Prevalence of Sub Clinical Mastitis and Associated Risk Factors in and around Gondar, Northern Ethiopia. International Journal of Animal health and Veterinary Advances 3 (6): 455-459.
[33]
Mohamed, A., Simeon, E., and Yemesrach, A. (2004): Dairy development in Ethiopia. International Food Policy Research Institute, EPTD Discussion Paper No. 123. Washington, DC, U.S.A.
[34]
Mulugeta, Y., and Wassie, M. (2013): Prevalence, risk factors and major bacteria causes of bovine mastitis in and around Wolaitasodo, Southern Ethiopia. African Jornal of Microbiological Research 7 (48): 5400-5405.
[35]
National Committee for clinical laboratory standards (1997): Performance standard for antibial disc and dilution susceptibility test for bacteria isolated from animals and humans, humans, approved standard, NCCLS document M31-a NCCLS, Villanova, PA.
[36]
Nibret, M., Tekle. H., Tewodros. F., Mersha, C., and Achenafi, M. (2012): Bovine mastitis and associated risk factors in small holder lactating dairy farms in Hawassa, Southern Ethiopia. Global Veterinaria, 9 (4): 441-446.
[37]
NMC, (1999): Current Concept in bovine mastitis National mastitis Council (NMC). 3rd. 1840 Wilsonblud, Arlinton, VA 22201.
[38]
Paulrud, C. O. (2005): Basic concepts of the bovine teat canal. Veterinary Research Communication, 9: 215–245.
[39]
Radostits, O. M., C. C. Gay, D. C. Blood and K. W. Hinchlif, (2007): Veterinary Medicine 9 Ed., Harcourt Ltd and London, Pp: 174-758.
[40]
Quinn, P. J., Markey, B. K., Carter, M. E., Donelly, W. J., Leonard, F. C. (2002). Veterinary Microbiologyand Microbial Disease. Blackwell Science Ltd, a Blackwell publishing Company. Pp: 326- 475.
[41]
Radostits, O., C. Gay, W. Hinchcliffand D. Constable (2007). Mastitis. In Veterinary Medicine: A Text book of disease of cattle, sheep, pigs, goats, and horses 10th edition, Ballier, Tindall, London. Pp: 674-762.
[42]
Riollet, C. and Rainard, P. (2006): innate immunity of the bovine mammary gland. Veterinary Research 37: 369–400.
[43]
Rehman, H. D., Sambyal, S., and Boxi, K. (1983): Incidence and etiology of subclinical mastitis in cows and buffaloes in Punja Agricultural university Ludhiana, India 20: 208-212. Sordillo, L. M., (2009): Current Concepts on Immunity and Mastitis. DCDS Advances in Dairy Technology 21: 111-119.
[44]
Standard veterinary laboratory diagnostic manual (2005): ministry of agriculture and rural development animal health department 2: 158-160.
[45]
Tsegai B (1997): Bovine mastitis in and around Bedele in zebu breed under village. DVM, Thesis submitted to the Faculty of Veterinary Medicine, Addis Ababa University, Ethiopia.
[46]
Vandepitte, J. and Verhaegen, J. (2003): Basic laboratory procedures in clinical bacteriology 2nd edition PP. 40-95.
[47]
Workineh, S., M. Bayleyeng, M. Mekonnen, H., and Potgieter, L. (2002): Prevalence and Etiology of Mastitis in Cows from Two Major Ethiopia Dairies. Tropical Animal Health and Production 34: 19-25.
[48]
Zerihun, T. (1996): A Study on Bovine Subclinical Mastitis at Stela Dairy Farm. DVM, Thesis submitted to the Faculty of veterinary Medicine, Addis Ababa University, Ethiopia.
[49]
Zeryhun, T., Aya, T., and Bayecha, R. (2013): Study on Prevalence, Bacterial Pathogens and Associated Risk Factors of Bovine Mastitis In Small Holder Dairy Farms in and Around Addis Ababa, Ethiopia. Journal of Animal and Plant Science, 23 (1): 50-55.
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